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transporter certified human hepatocytes (phh)  (BioIVT Inc)

 
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    BioIVT Inc transporter certified human hepatocytes (phh)
    (A) Illustration of the in vitro , <t>hepatic</t> <t>cell-based</t> differential gene expression assay design. (B) THRB and THRA RNA levels were quantified by RT-qPCR in HepG2 (n = 3), Huh-7, (n = 3), and <t>PHH</t> (n = 5) cells. Mean RQ values ± SEM are reported with means annotated within the bars. (C) Huh-7 cells were treated with increasing doses of T3 (n = 2), GC-1 (n = 2), or MGL-3196 (n = 2) for 24 hrs. ANGPLT4 , CPT1A , and DIO1 RNA levels were quantified by RT-qPCR and dose-response curves were generated for each gene-compound combination. Mean EC 50 values (red bar) and individual replicate EC 50 values (black symbols) are reported. (D) Huh-7 cells were treated with increasing doses of T3 (black), GC-1 (red), MGL-3196 (green), VK2809A (solid blue), or VK2809 (dashed blue) for 24 hrs. CPT1A RNA levels were quantified by RT-qPCR. Representative mean RQ values at each compound concentration and fitted dose-response curves are reported. (E) PHH were treated with increasing doses of T3 (black), GC-1 (red), MGL-3196 (green), VK2809A (solid blue), or VK2809 (dashed blue) for 24 hrs. THRSP RNA levels were quantified by RT-qPCR. Representative mean RQ values at each compound concentration and fitted dose-response curves are reported. (F) EC 50 values for every test compound were calculated from dose-response curves generated from the TR-FRET THRβ, luciferase (Luc) reporter THRβ, Huh-7 differential gene expression (RQ), and PHH RQ assays (data reported in Tables and ). Mean EC 50 values ± SEM are reported.
    Transporter Certified Human Hepatocytes (Phh), supplied by BioIVT Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/transporter certified human hepatocytes (phh)/product/BioIVT Inc
    Average 90 stars, based on 1 article reviews
    transporter certified human hepatocytes (phh) - by Bioz Stars, 2026-02
    90/100 stars

    Images

    1) Product Images from "Regulation of gene transcription by thyroid hormone receptor β agonists in clinical development for the treatment of non-alcoholic steatohepatitis (NASH)"

    Article Title: Regulation of gene transcription by thyroid hormone receptor β agonists in clinical development for the treatment of non-alcoholic steatohepatitis (NASH)

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0240338

    (A) Illustration of the in vitro , hepatic cell-based differential gene expression assay design. (B) THRB and THRA RNA levels were quantified by RT-qPCR in HepG2 (n = 3), Huh-7, (n = 3), and PHH (n = 5) cells. Mean RQ values ± SEM are reported with means annotated within the bars. (C) Huh-7 cells were treated with increasing doses of T3 (n = 2), GC-1 (n = 2), or MGL-3196 (n = 2) for 24 hrs. ANGPLT4 , CPT1A , and DIO1 RNA levels were quantified by RT-qPCR and dose-response curves were generated for each gene-compound combination. Mean EC 50 values (red bar) and individual replicate EC 50 values (black symbols) are reported. (D) Huh-7 cells were treated with increasing doses of T3 (black), GC-1 (red), MGL-3196 (green), VK2809A (solid blue), or VK2809 (dashed blue) for 24 hrs. CPT1A RNA levels were quantified by RT-qPCR. Representative mean RQ values at each compound concentration and fitted dose-response curves are reported. (E) PHH were treated with increasing doses of T3 (black), GC-1 (red), MGL-3196 (green), VK2809A (solid blue), or VK2809 (dashed blue) for 24 hrs. THRSP RNA levels were quantified by RT-qPCR. Representative mean RQ values at each compound concentration and fitted dose-response curves are reported. (F) EC 50 values for every test compound were calculated from dose-response curves generated from the TR-FRET THRβ, luciferase (Luc) reporter THRβ, Huh-7 differential gene expression (RQ), and PHH RQ assays (data reported in Tables and ). Mean EC 50 values ± SEM are reported.
    Figure Legend Snippet: (A) Illustration of the in vitro , hepatic cell-based differential gene expression assay design. (B) THRB and THRA RNA levels were quantified by RT-qPCR in HepG2 (n = 3), Huh-7, (n = 3), and PHH (n = 5) cells. Mean RQ values ± SEM are reported with means annotated within the bars. (C) Huh-7 cells were treated with increasing doses of T3 (n = 2), GC-1 (n = 2), or MGL-3196 (n = 2) for 24 hrs. ANGPLT4 , CPT1A , and DIO1 RNA levels were quantified by RT-qPCR and dose-response curves were generated for each gene-compound combination. Mean EC 50 values (red bar) and individual replicate EC 50 values (black symbols) are reported. (D) Huh-7 cells were treated with increasing doses of T3 (black), GC-1 (red), MGL-3196 (green), VK2809A (solid blue), or VK2809 (dashed blue) for 24 hrs. CPT1A RNA levels were quantified by RT-qPCR. Representative mean RQ values at each compound concentration and fitted dose-response curves are reported. (E) PHH were treated with increasing doses of T3 (black), GC-1 (red), MGL-3196 (green), VK2809A (solid blue), or VK2809 (dashed blue) for 24 hrs. THRSP RNA levels were quantified by RT-qPCR. Representative mean RQ values at each compound concentration and fitted dose-response curves are reported. (F) EC 50 values for every test compound were calculated from dose-response curves generated from the TR-FRET THRβ, luciferase (Luc) reporter THRβ, Huh-7 differential gene expression (RQ), and PHH RQ assays (data reported in Tables and ). Mean EC 50 values ± SEM are reported.

    Techniques Used: In Vitro, Gene Expression, Quantitative RT-PCR, Generated, Concentration Assay, Luciferase



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    BioIVT Inc transporter certified human hepatocytes (phh)
    (A) Illustration of the in vitro , <t>hepatic</t> <t>cell-based</t> differential gene expression assay design. (B) THRB and THRA RNA levels were quantified by RT-qPCR in HepG2 (n = 3), Huh-7, (n = 3), and <t>PHH</t> (n = 5) cells. Mean RQ values ± SEM are reported with means annotated within the bars. (C) Huh-7 cells were treated with increasing doses of T3 (n = 2), GC-1 (n = 2), or MGL-3196 (n = 2) for 24 hrs. ANGPLT4 , CPT1A , and DIO1 RNA levels were quantified by RT-qPCR and dose-response curves were generated for each gene-compound combination. Mean EC 50 values (red bar) and individual replicate EC 50 values (black symbols) are reported. (D) Huh-7 cells were treated with increasing doses of T3 (black), GC-1 (red), MGL-3196 (green), VK2809A (solid blue), or VK2809 (dashed blue) for 24 hrs. CPT1A RNA levels were quantified by RT-qPCR. Representative mean RQ values at each compound concentration and fitted dose-response curves are reported. (E) PHH were treated with increasing doses of T3 (black), GC-1 (red), MGL-3196 (green), VK2809A (solid blue), or VK2809 (dashed blue) for 24 hrs. THRSP RNA levels were quantified by RT-qPCR. Representative mean RQ values at each compound concentration and fitted dose-response curves are reported. (F) EC 50 values for every test compound were calculated from dose-response curves generated from the TR-FRET THRβ, luciferase (Luc) reporter THRβ, Huh-7 differential gene expression (RQ), and PHH RQ assays (data reported in Tables and ). Mean EC 50 values ± SEM are reported.
    Transporter Certified Human Hepatocytes (Phh), supplied by BioIVT Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/transporter certified human hepatocytes (phh)/product/BioIVT Inc
    Average 90 stars, based on 1 article reviews
    transporter certified human hepatocytes (phh) - by Bioz Stars, 2026-02
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    (A) Illustration of the in vitro , hepatic cell-based differential gene expression assay design. (B) THRB and THRA RNA levels were quantified by RT-qPCR in HepG2 (n = 3), Huh-7, (n = 3), and PHH (n = 5) cells. Mean RQ values ± SEM are reported with means annotated within the bars. (C) Huh-7 cells were treated with increasing doses of T3 (n = 2), GC-1 (n = 2), or MGL-3196 (n = 2) for 24 hrs. ANGPLT4 , CPT1A , and DIO1 RNA levels were quantified by RT-qPCR and dose-response curves were generated for each gene-compound combination. Mean EC 50 values (red bar) and individual replicate EC 50 values (black symbols) are reported. (D) Huh-7 cells were treated with increasing doses of T3 (black), GC-1 (red), MGL-3196 (green), VK2809A (solid blue), or VK2809 (dashed blue) for 24 hrs. CPT1A RNA levels were quantified by RT-qPCR. Representative mean RQ values at each compound concentration and fitted dose-response curves are reported. (E) PHH were treated with increasing doses of T3 (black), GC-1 (red), MGL-3196 (green), VK2809A (solid blue), or VK2809 (dashed blue) for 24 hrs. THRSP RNA levels were quantified by RT-qPCR. Representative mean RQ values at each compound concentration and fitted dose-response curves are reported. (F) EC 50 values for every test compound were calculated from dose-response curves generated from the TR-FRET THRβ, luciferase (Luc) reporter THRβ, Huh-7 differential gene expression (RQ), and PHH RQ assays (data reported in Tables and ). Mean EC 50 values ± SEM are reported.

    Journal: PLoS ONE

    Article Title: Regulation of gene transcription by thyroid hormone receptor β agonists in clinical development for the treatment of non-alcoholic steatohepatitis (NASH)

    doi: 10.1371/journal.pone.0240338

    Figure Lengend Snippet: (A) Illustration of the in vitro , hepatic cell-based differential gene expression assay design. (B) THRB and THRA RNA levels were quantified by RT-qPCR in HepG2 (n = 3), Huh-7, (n = 3), and PHH (n = 5) cells. Mean RQ values ± SEM are reported with means annotated within the bars. (C) Huh-7 cells were treated with increasing doses of T3 (n = 2), GC-1 (n = 2), or MGL-3196 (n = 2) for 24 hrs. ANGPLT4 , CPT1A , and DIO1 RNA levels were quantified by RT-qPCR and dose-response curves were generated for each gene-compound combination. Mean EC 50 values (red bar) and individual replicate EC 50 values (black symbols) are reported. (D) Huh-7 cells were treated with increasing doses of T3 (black), GC-1 (red), MGL-3196 (green), VK2809A (solid blue), or VK2809 (dashed blue) for 24 hrs. CPT1A RNA levels were quantified by RT-qPCR. Representative mean RQ values at each compound concentration and fitted dose-response curves are reported. (E) PHH were treated with increasing doses of T3 (black), GC-1 (red), MGL-3196 (green), VK2809A (solid blue), or VK2809 (dashed blue) for 24 hrs. THRSP RNA levels were quantified by RT-qPCR. Representative mean RQ values at each compound concentration and fitted dose-response curves are reported. (F) EC 50 values for every test compound were calculated from dose-response curves generated from the TR-FRET THRβ, luciferase (Luc) reporter THRβ, Huh-7 differential gene expression (RQ), and PHH RQ assays (data reported in Tables and ). Mean EC 50 values ± SEM are reported.

    Article Snippet: Transporter certified human hepatocytes (PHH) were obtained from BioIVT (Lot: JEL, F00995-TCERT).

    Techniques: In Vitro, Gene Expression, Quantitative RT-PCR, Generated, Concentration Assay, Luciferase